ID   U87107     standard; DNA; SYN; 8840 BP.
XX
AC   U87107;
XX
SV   U87107.1
XX
DT   15-OCT-1997 (Rel. 52, Created)
DT   15-OCT-1997 (Rel. 52, Last updated, Version 4)
XX
DE   Cloning vector pAL-F insertion sequence IS1 galactokinase (galK),
DE   aminoglycoside 3'-phosphotransferase (kn), beta-galactosidase (lacZ), small
DE   ribosomal protein and beta-lactamase (Ap) genes, complete cds.
XX
KW   .
XX
OS   Cloning vector pAL-F
OC   artificial sequence; vectors.
XX
RN   [1]
RP   1-8840
RA   Ahmed A., Podemski L.;
RT   "Use of ordered deletions in genome sequencing";
RL   Gene 197:367-373(1997).
XX
RN   [2]
RP   1-8840
RA   Ahmed A.;
RT   ;
RL   Submitted (27-JAN-1997) to the EMBL/GenBank/DDBJ databases.
RL   Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9,
RL   Canada
XX
DR   REMTREMBL; AAC53713; AAC53713.
DR   REMTREMBL; AAC53714; AAC53714.
DR   REMTREMBL; AAC53715; AAC53715.
DR   REMTREMBL; AAC53716; AAC53716.
DR   REMTREMBL; AAC53717; AAC53717.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..8840
FT                   /db_xref="taxon:56954"
FT                   /organism="Cloning vector pAL-F"
FT                   /insertion_seq="IS1"
FT                   /specific_host="Escherichia coli"
FT   CDS             complement(933..2081)
FT                   /codon_start=1
FT                   /db_xref="REMTREMBL:AAC53713"
FT                   /transl_table=11
FT                   /gene="galK"
FT                   /product="galactokinase"
FT                   /protein_id="AAC53713.1"
FT                   /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLP
FT                   CAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQ
FT                   LRNNSFGGVDMVDHGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAEN
FT                   QFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSE
FT                   YNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASA
FT                   LEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCI
FT                   VALIPEELVPAVQQRVAEQYEAKTGIKETFYVCKPSQGAGQC"
FT   CDS             2875..3669
FT                   /codon_start=1
FT                   /db_xref="REMTREMBL:AAC53714"
FT                   /transl_table=11
FT                   /gene="kn"
FT                   /function="kanamycin resistance"
FT                   /product="aminoglycoside 3'-phosphotransferase"
FT                   /protein_id="AAC53714.1"
FT                   /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
FT                   VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
FT                   SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
FT                   GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
FT                   LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
FT   CDS             3978..4442
FT                   /codon_start=1
FT                   /db_xref="REMTREMBL:AAC53715"
FT                   /note="alpha fragment"
FT                   /transl_table=11
FT                   /gene="lacZ"
FT                   /product="beta-galactosidase"
FT                   /protein_id="AAC53715.1"
FT                   /translation="MTMITNSCRRPRARGTVDGSLIKFKSPARRARVDGTTSLALAVVL
FT                   QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLALYALMQF
FT                   LCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWIDPAEFYADQ"
FT   CDS             complement(4555..4929)
FT                   /codon_start=1
FT                   /db_xref="REMTREMBL:AAC53716"
FT                   /transl_table=11
FT                   /function="streptomycin sensitivity"
FT                   /product="small ribosomal protein"
FT                   /protein_id="AAC53716.1"
FT                   /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP
FT                   NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC
FT                   SGVKDRKQARSKYGVKRPKA"
FT   CDS             complement(7087..7947)
FT                   /codon_start=1
FT                   /db_xref="REMTREMBL:AAC53717"
FT                   /transl_table=11
FT                   /gene="Ap"
FT                   /function="ampicillin resistance"
FT                   /product="beta-lactamase"
FT                   /protein_id="AAC53717.1"
FT                   /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
FT                   ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
FT                   PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
FT                   EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
FT                   LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
FT                   LIKHW"
XX
SQ   Sequence 8840 BP; 2068 A; 2288 C; 2319 G; 2165 T; 0 other;
     caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa        60
     gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg       120
     ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct       180
     caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg       240
     ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg       300
     tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt       360
     cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat       420
     aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc       480
     gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca       540
     taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa       600
     gcgaggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg       660
     gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac       720
     tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat       780
     gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag       840
     ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatcaaca gtcggtacgg       900
     ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt       960
     ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacacgt      1020
     tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg      1080
     ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga      1140
     gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc      1200
     atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg      1260
     cgggcgtttt cagtcagtat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc      1320
     gcaacagcgt tgaactcttc aatggtgaca tcacgcaggg ctggctgctg gaagaaacgc      1380
     gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg      1440
     aagttactgt tgatgatgac gacagccaca cctttgggca tggaaactgc tttggtcccc      1500
     agtgagcggc aatcgatcag caaggcatga tctttcttgc cgagcgcgga aattagctga      1560
     tccatgatcc cgcagttaca gcctacaaac tggttttctg cttcctgacc gttaagcgcg      1620
     atttgtgcgc cgtccagcgg cagatgataa agctgctgca atacggttcc gaccgcgact      1680
     tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgtg atcaaccatg      1740
     tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag      1800
     ttagcccatt gatagttttc atgtgcgaca atgggcgcat cgagggaaaa ctcgtcgagc      1860
     tgattttcat aatcggctgc catcacgcga actttacggt catcgcgtgg tgcacaactg      1920
     atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt      1980
     tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat      2040
     gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg      2100
     cgacggccta cagcaacctg ggcgatgtat ggcatcaggt tattcggaat gccttgcgga      2160
     tcttcgccca tatcgcccga cggatgcgcg ccaaccgggt tgaagtagcg cacgagggca      2220
     atgctccagt ccggctgggc tttttgcaga tcggtgagga tctgttccac catcagcttg      2280
     cttttgccgt aagggctttg cggtgtgccg gtcgggaagc tataatgcgg tagtttatca      2340
     cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc      2400
     atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg      2460
     ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta      2520
     gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag      2580
     aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg      2640
     acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga      2700
     tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc      2760
     tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc      2820
     tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt      2880
     gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat      2940
     gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag      3000
     gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaagac      3060
     gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac      3120
     gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc      3180
     ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg      3240
     ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag      3300
     cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat      3360
     caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc cgacggcgag      3420
     gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc      3480
     ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg      3540
     ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg      3600
     ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag      3660
     ttcttctgag cgggactctg gggttcgcga tgataagctg tcaaacatga gaattacaac      3720
     ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc      3780
     tagaaatatt ttatctgatt cgattcatta atgcagctgg cacgacaggt ttcccgactg      3840
     gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca      3900
     ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt      3960
     tcacacagga aacagctatg accatgatta cgaattcctg caggcggccg cgagctcgag      4020
     gtaccgtcga cggatcctta attaaattta aatcaccggc gcgccgagct cgagtcgacg      4080
     gtaccacaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg      4140
     ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag      4200
     aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgata      4260
     agctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg      4320
     accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg      4380
     cgatcatggc gaccacaccc gtcctgtgga tcgatccggc agaattttac gctgaccaat      4440
     gacgcgacga cgtggcatgg aaatactccg ttgttaattc aggattgtcc aaaactctac      4500
     gagtttagtt tgacatttaa gttaaaacgt ttagccttac ttaacggaga accattaagc      4560
     cttaggacgc ttcacgccat acttggaacg agcctgctta cggtctttaa cgccggagca      4620
     gtcaagcgca ccacgtacgg tgtggtaacg aacacccggg aggtctttaa cacgaccgcc      4680
     acggatcagg atcacggagt gctcctgcag gttgtgacct tcaccaccga tgtaggaagt      4740
     cacttcgaaa ccgttagtca gacgaacacg gcatacttta cgcagcgcgg agttcggttt      4800
     tttaggagtg gtagtatata cacgagtaca tacgccacgt ttttgcgggc atgcttccag      4860
     cgcaggcacg ttgcttttcg caactttgcg agcacgtggt ttgcgtacca gctggttaac      4920
     tgttgccatt aaatagctcc tggttttagc ttttgcttcg taaacacgta ataaaacgtc      4980
     ctcacacaat atgaggacgc cgaattttag ggcgatgccg aaaaggtgtc aagaaatata      5040
     caacgatccc gccatcatca ccaggccatc tggctggggt gcttaaccgt aagtctgacg      5100
     aaatcagtat agtcaatgag aatgatgtcg ttcgaaattt gaccagtcaa acccaaacca      5160
     acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct      5220
     cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc      5280
     ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa      5340
     cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg      5400
     gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc      5460
     ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga      5520
     agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt      5580
     gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga      5640
     gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg      5700
     aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc      5760
     agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct      5820
     gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg      5880
     atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag      5940
     cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg      6000
     gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc      6060
     atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt      6120
     aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc      6180
     ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac      6240
     agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa      6300
     ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca      6360
     caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc      6420
     gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata      6480
     cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta      6540
     tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca      6600
     gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga      6660
     cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg      6720
     tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg      6780
     tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg      6840
     caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag      6900
     aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa      6960
     cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat      7020
     ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc      7080
     tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc      7140
     atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc      7200
     tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc      7260
     aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc      7320
     catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt      7380
     gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc      7440
     ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa      7500
     aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt      7560
     atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg      7620
     cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc      7680
     gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa      7740
     agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt      7800
     gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt      7860
     caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag      7920
     ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta      7980
     tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat      8040
     aggggttccg cgcacatttc cccgaaaagt gcggtaatga ctccaactta ttgatagtgt      8100
     tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac      8160
     agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt      8220
     cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc      8280
     ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc      8340
     cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg      8400
     tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc ccactgttcg      8460
     tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc      8520
     ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg      8580
     ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt      8640
     gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc      8700
     gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg      8760
     gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc      8820
     agtaagttgg cagcatcacc                                                  8840
//
