LOCUS       pBAD30       4923 bp    DNA   circular            
SOURCE      
  ORGANISM  
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTREPLTYPE|Phagemid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     rep_origin      1..843
                     /vntifkey="33"
                     /label=P15A_ORI
     CDS             complement(1082..1960)
                     /vntifkey="4"
                     /label=araC
                     /note="araC regulator of the arabinose BAD promoter"
     misc_feature    1989..2006
                     /vntifkey="21"
                     /label=operator\O2
                     /note="operator O2"
     promoter        complement(2111..2139)
                     /vntifkey="30"
                     /label=araC\promoter
                     /note="araC promoter"
     misc_feature    2147..2168
                     /vntifkey="21"
                     /label=operator\O1
                     /note="operator O1"
     misc_feature    2190..2203
                     /vntifkey="21"
                     /label=CAP\site
                     /note="CAP site"
     misc_feature    2199..2237
                     /vntifkey="21"
                     /label=operator\I2\and\I1
                     /note="operator I2 + I1"
     promoter        2236..2263
                     /vntifkey="30"
                     /label=arabinose\BAD\promoter
                     /note="arabinose pBAD promoter"
     misc_feature    2286..2348
                     /vntifkey="21"
                     /label=MCS
                     /note="multiple cloning site"
     terminator      2349..2774
                     /vntifkey="43"
                     /label=rrnB\T1\T2
     promoter        2829..2835
                     /vntifkey="30"
                     /label=P(BLA)
                     /note="bla P3 promoter"
     CDS             2867..3730
                     /vntifkey="4"
                     /label=AP(R)
     rep_origin      3766..4224
                     /vntifkey="33"
                     /label=M13\ORI
                     /note="M13 origin"
BASE COUNT     1240 a      1274 c      1181 g      1228 t 
ORIGIN
        1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct 
       61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata 
      121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa 
      181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa 
      241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg 
      301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt 
      361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca 
      421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca 
      481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta 
      541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca 
      601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac 
      661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt 
      721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca 
      781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata 
      841 tttgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 
      901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 
      961 gatctgctca tgtttgacag cttatcatcg atgcataatg tgcctgtcaa atggacgaag 
     1021 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca 
     1081 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc 
     1141 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac 
     1201 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct 
     1261 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa 
     1321 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat 
     1381 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg 
     1441 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat 
     1501 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa 
     1561 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa 
     1621 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact 
     1681 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga 
     1741 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct 
     1801 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa 
     1861 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg 
     1921 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat 
     1981 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact 
     2041 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg 
     2101 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc 
     2161 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 
     2221 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt 
     2281 tttgggctag cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat 
     2341 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 
     2401 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 
     2461 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 
     2521 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac 
     2581 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 
     2641 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 
     2701 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 
     2761 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     2821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     2881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     2941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     3001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     3061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 
     3121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     3181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     3241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     3301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     3361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc 
     3421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     3541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     3721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac gcgccctgta 
     3781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     3841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     3901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     3961 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat 
     4021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     4081 aaacttgaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc 
     4141 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 
     4201 acaaaatatt aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc 
     4261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     4321 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     4381 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     4441 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 
     4501 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     4561 ttaccagtca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg 
     4621 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt 
     4681 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca 
     4741 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca 
     4801 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca 
     4861 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 
     4921 atg 
//
