METATOOL OUTPUT Version 3.5 (28.03.2001) C:\METATOOL\META35~1.EXE

INPUT FILE: EXAMPLE6.TXT

INTERNAL METABOLITES: 13
REACTIONS: 6

  4 external H2O
  3 external H2O2
  3 external O2
  2 int      RCH2NH2
  2 int      RCHO
  2 int      NH3
  1 int      Donor
  1 int      oxidized_donor
  1 int      4-Hydroxyphenylpyruvate
  1 int      homogentisate
  1 int      CO2
  1 int      An_aldehyde
  1 int      NAD(P)+
  1 int      an_acid
  1 int      NAD(P)H
  1 int      H+
 16 metabolites,  26 is the summarized frequency

edges	frequency of nodes
  0	  0
  1	 10
  2	  3
  3	  2
  4	  1

STOICHIOMETRIC MATRIX
 
 matrix dimension r13 x c6
 0 -1  0  0  0  0 
 0  1  0  0  0  0 
 0  0 -1  0  0  0 
 0  0  1  0  0  0 
 0  0  1  0  0  0 
-1  0  0  0  0  0 
-1  0  0  0  0  0 
 1  0  0  0  0  0 
 1  0  0  0  0  0 
 1  0  0  0  0  0 
 0  0  0  0 -1 -1 
 0  0  0  0  1  1 
 0  0  0  0  1  1 
The following line indicates reversible (0) and irreversible reactions (1)
 0  1  1  1  1  1 
rows and columns are sorted as declared in the inputfile

NOT BALANCED INTERNAL METABOLITES
Donor	oxidized_donor	4-Hydroxyphenylpyruvate	homogentisate	CO2	An_aldehyde	NAD(P)+	an_acid	NAD(P)H	H+	

KERNEL
 
 matrix dimension r2 x c6
 0  0  0  1  0  0 
 0  0  0  0  1 -1 
6 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section.

 enzymes

 1:	 1.2.1.5 irreversible
 2:	 1.4.3.4 -1.4.3.6 irreversible

 overall reaction
 - not found -


The remaining overall reactions perform no net transformation of external metabolites.

SUBSETS OF REACTIONS
 
 matrix dimension r2 x c6
 0  0  0  1  0  0 
 0  0  0  0 -1  1 
6 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section.

 enzymes

 1:	 1.2.1.5 irreversible
 2:	 -1.4.3.4 1.4.3.6 irreversible

 overall reaction
 - not found -


The remaining overall reactions perform no net transformation of external metabolites.

!!! Subset 2 with contradictory irreversibility constraints, please check your model.
    Enzymes of that subset are cancelled for further calculations.

REDUCED SYSTEM with 13 branch point metabolites in 2 reactions (columns)
 
 matrix dimension r0 x c2
The following line indicates reversible (0) and irreversible reactions (1)
 1  1 

-> Branch metabolites are : 
met                 	cons	built	reactions

-> No branch metabolites are : 
met                 	cons	built	reactions
Donor               	1	0	1	i
oxidized_donor      	0	1	1	i
4-Hydroxyphenylpyruvate	1	0	1	i
homogentisate       	0	1	1	i
CO2                 	0	1	1	i
An_aldehyde         	1	0	1	r
NAD(P)+             	1	0	1	r
an_acid             	0	1	1	r
NAD(P)H             	0	1	1	r
H+                  	0	1	1	r
RCH2NH2             	2	0	2	ii
RCHO                	0	2	2	ii
NH3                 	0	2	2	ii

CONVEX BASIS
 
 matrix dimension r1 x c6
 0  0  0  1  0  0 

 enzymes

 1:	 1.2.1.5 irreversible

 overall reaction
 - not found -


The remaining overall reactions perform no net transformation of external metabolites.

CONSERVATION RELATIONS
 
 matrix dimension r9 x c13
 1  1  0  0  0  0  0  0  0  0  0  0  0 
 0  0  1  1  0  0  0  0  0  0  0  0  0 
 0  0  1  0  1  0  0  0  0  0  0  0  0 
 0  0  0  0  0  1 -1  0  0  0  0  0  0 
 0  0  0  0  0  1  0  1  0  0  0  0  0 
 0  0  0  0  0  1  0  0  1  0  0  0  0 
 0  0  0  0  0  1  0  0  0  1  0  0  0 
 0  0  0  0  0  0  0  0  0  0  1  1  0 
 0  0  0  0  0  0  0  0  0  0  1  0  1 
 1 :	Donor + oxidized_donor = const
 2 :	4-Hydroxyphenylpyruvate + homogentisate = const
 3 :	4-Hydroxyphenylpyruvate + CO2 = const
 4 :	An_aldehyde + NAD(P)+ = const
 5 :	An_aldehyde + an_acid = const
 6 :	An_aldehyde + NAD(P)H = const
 7 :	An_aldehyde + H+ = const
 8 :	RCH2NH2 + RCHO = const
 9 :	RCH2NH2 + NH3 = const

ELEMENTARY MODES
 
 matrix dimension r1 x c6
 0  0  0  1  0  0 
6 reactions (columns) are sorted in the same order as in the ENZREV ENZIRREV section.

 enzymes

 1:	 1.2.1.5 irreversible

 overall reaction
 - not found -


The remaining overall reactions perform no net transformation of external metabolites.

The elementary modes ARE EQUAL to convex basis.
