| I-CreI Homing endonuclease |

NOTE: Homing endonucleases do not really have recognition sequences in the way that restriction enzymes do. The recognition sequence listed is one site that is known to be recognized and cleaved. In general, single base changes merely change the efficiency of cleavage and the precise boundary of required bases is not known.

Recognition Sequence:

     C T G G G T T C A A A A C G T C G T G A^G A C A G T T T G G 0
     G A C C C A A G T T T T G C A G^C A C T C T G T C A A A C C 0

REBASE enzyme #: 2540 Prototype: I-CreI Source: J.-D. Rochaix Microorganism: Chlamydomonas reinhardtii Growth Temperature: 26 ° Enzyme gene cloned. Enzyme gene sequenced. Crystal data present. Comment: Insertion site CGT^CGT Entered: Jan 1 1979 ... Modified: Sep 9 1999

# sites on Adeno2: 0 Lambda: 0 pBR322: 0 PhiX174: 0 SV40: 0

Related References... (sorted by date) (sorted by authors) | NOT commercially available | Sequence Data... | Crystal Data...

|  | 07/13/2000 Dr. Richard J. Roberts and Dana Macelis |  |