BLASTX 2.2.26+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: NCBI Protein Reference Sequences
           11,879,989 sequences; 4,140,237,112 total letters



Query= gi|356995852:1-490 Mus musculus POU domain, class 5, transcription
factor 1 (Pou5f1), transcript variant 1, mRNA

Length=490
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

ref|NP_038661.2|  POU domain, class 5, transcription factor 1 iso...   192    4e-57
ref|NP_001009178.1|  POU class 5 homeobox 1 [Rattus norvegicus]        179    3e-52
ref|XP_003505911.1|  PREDICTED: POU domain, class 5, transcriptio...   147    7e-40
ref|XP_001490158.1|  PREDICTED: POU domain, class 5, transcriptio...   141    8e-38
ref|XP_001135162.1|  PREDICTED: POU domain, class 5, transcriptio...   138    1e-36
ref|NP_002692.2|  POU domain, class 5, transcription factor 1 iso...   138    2e-36
ref|XP_002809144.1|  PREDICTED: POU domain, class 5, transcriptio...   137    2e-36
ref|XP_528230.1|  PREDICTED: putative POU domain, class 5, transc...   133    1e-34
ref|XP_002746363.1|  PREDICTED: POU domain, class 5, transcriptio...   133    1e-34
ref|NP_001108427.1|  POU domain, class 5, transcription factor 1 ...   133    1e-34
ref|NP_001106531.1|  POU domain, class 5, transcription factor 1 ...   133    1e-34
ref|XP_002752527.1|  PREDICTED: POU domain, class 5, transcriptio...   132    2e-34
ref|NP_001166912.1|  POU domain, class 5, transcription factor 1 ...   131    6e-34
ref|NP_777005.1|  POU domain, class 5, transcription factor 1 [Bo...   131    6e-34
ref|NP_001153014.1|  putative POU domain, class 5, transcription ...   128    6e-33
ref|NP_001093427.1|  POU domain, class 5, transcription factor 1 ...   127    2e-32
ref|XP_538830.1|  PREDICTED: POU domain, class 5, transcription f...   119    1e-29
ref|XP_003422494.1|  PREDICTED: POU domain, class 5, transcriptio...   118    4e-29
ref|XP_003474038.1|  PREDICTED: POU domain, class 5, transcriptio...   117    7e-29
ref|XP_003271964.1|  PREDICTED: LOW QUALITY PROTEIN: POU domain, ...   111    7e-27


>ref|NP_038661.2| POU domain, class 5, transcription factor 1 isoform 1 [Mus musculus]
Length=352

 Score =  192 bits (487),  Expect = 4e-57
 Identities = 140/140 (100%), Positives = 140/140 (100%), Gaps = 0/140 (0%)
 Frame = +3

Query  69   MAGHLAsdfafspppgggdgsagLEPGWVDPRTWLSFQgppggpgigpgSEVLGISPCPP  248
            MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPP
Sbjct  1    MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPP  60

Query  249  AYEFCGGMAYCgpqvglglvpqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE  428
            AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE
Sbjct  61   AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE  120

Query  429  KVEPTPEESQDMKALQKELE  488
            KVEPTPEESQDMKALQKELE
Sbjct  121  KVEPTPEESQDMKALQKELE  140


>ref|NP_001009178.1| POU class 5 homeobox 1 [Rattus norvegicus]
Length=352

 Score =  179 bits (454),  Expect = 3e-52
 Identities = 133/140 (95%), Positives = 135/140 (96%), Gaps = 0/140 (0%)
 Frame = +3

Query  69   MAGHLAsdfafspppgggdgsagLEPGWVDPRTWLSFQgppggpgigpgSEVLGISPCPP  248
            MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPP GPGIGPGSEVLGISPCPP
Sbjct  1    MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPSGPGIGPGSEVLGISPCPP  60

Query  249  AYEFCGGMAYCgpqvglglvpqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE  428
            AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG SS PC  RP+AVKLE
Sbjct  61   AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGASSGPCTARPSAVKLE  120

Query  429  KVEPTPEESQDMKALQKELE  488
            KVEP+PEESQDMKALQKELE
Sbjct  121  KVEPSPEESQDMKALQKELE  140


>ref|XP_003505911.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Cricetulus 
griseus]
Length=358

 Score =  147 bits (370),  Expect = 7e-40
 Identities = 120/145 (83%), Positives = 128/145 (88%), Gaps = 5/145 (3%)
 Frame = +3

Query  69   MAGHLAsdfafspppgggdgsagLEPGWVDPRTWLSFQgppggpgigpg----SEVLGIS  236
            MAGHLASDFAFSPPPGGGDGS GLEPGWVDPRTWLSFQGPPGGPGIGPG    SEVLGIS
Sbjct  1    MAGHLASDFAFSPPPGGGDGSGGLEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVLGIS  60

Query  237  PCPPAYEFCGGMAYCgpqvglglvpqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNA  416
            PCPP YEFCGG+AYCGPQVGLGLVP VG+ET QPEGQ+GA VE++SE +S  PC  RP  
Sbjct  61   PCPPPYEFCGGVAYCGPQVGLGLVPPVGLETSQPEGQSGAGVENDSEESSPGPCTARPIV  120

Query  417  -VKLEKVEPTPEESQDMKALQKELE  488
             VKLEKVEP+PEESQD+KALQKELE
Sbjct  121  PVKLEKVEPSPEESQDVKALQKELE  145


>ref|XP_001490158.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Equus 
caballus]
Length=360

 Score =  141 bits (356),  Expect = 8e-38
 Identities = 93/122 (76%), Positives = 101/122 (83%), Gaps = 6/122 (5%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            EPGWVDPRTWLSFQGPP G GIGPG    +EV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct  26   EPGWVDPRTWLSFQGPPSGSGIGPGVGPGAEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE  482
            PQ  +ET QPEG+AGARVESNSEG S EPCA  P AVK+  EK+E  PEESQD+KALQK+
Sbjct  86   PQGSLETSQPEGEAGARVESNSEGASPEPCAAPPGAVKVDKEKLEQNPEESQDIKALQKD  145

Query  483  LE  488
            LE
Sbjct  146  LE  147


>ref|XP_001135162.1| PREDICTED: POU domain, class 5, transcription factor 1 isoform 
2 [Pan troglodytes]
Length=359

 Score =  138 bits (348),  Expect = 1e-36
 Identities = 95/121 (79%), Positives = 101/121 (83%), Gaps = 5/121 (4%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            EPGWVDPRTWLSFQGPPGGPGIGPG    SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct  26   EPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE-KVEPTPEESQDMKALQKEL  485
            PQ G+ET QPEG+AG  VESNS+G S EPC   P AVKLE K+E  PEESQD+KALQKEL
Sbjct  86   PQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKKLEQNPEESQDIKALQKEL  145

Query  486  E  488
            E
Sbjct  146  E  146


>ref|NP_002692.2| POU domain, class 5, transcription factor 1 isoform 1 [Homo sapiens]
 ref|NP_001238970.1| POU domain, class 5, transcription factor 1 [Pan troglodytes]
Length=360

 Score =  138 bits (347),  Expect = 2e-36
 Identities = 95/122 (78%), Positives = 101/122 (83%), Gaps = 6/122 (5%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            EPGWVDPRTWLSFQGPPGGPGIGPG    SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct  26   EPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE  482
            PQ G+ET QPEG+AG  VESNS+G S EPC   P AVKL  EK+E  PEESQD+KALQKE
Sbjct  86   PQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKE  145

Query  483  LE  488
            LE
Sbjct  146  LE  147


>ref|XP_002809144.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Pongo 
abelii]
Length=360

 Score =  137 bits (346),  Expect = 2e-36
 Identities = 94/122 (77%), Positives = 100/122 (82%), Gaps = 6/122 (5%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            EPGWVDPRTWLSFQGPPGGPGIGPG    SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct  26   EPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE  482
            PQ  +ET QPEG+AG  VESNS+G S EPC   P AVKL  EK+E  PEESQD+KALQKE
Sbjct  86   PQGSLETSQPEGEAGVGVESNSDGASPEPCTVPPGAVKLEKEKLEQNPEESQDIKALQKE  145

Query  483  LE  488
            LE
Sbjct  146  LE  147


>ref|XP_528230.1| PREDICTED: putative POU domain, class 5, transcription factor 
1B [Pan troglodytes]
Length=359

 Score =  133 bits (334),  Expect = 1e-34
 Identities = 93/122 (76%), Positives = 98/122 (80%), Gaps = 6/122 (5%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            EPGWVDP TWL FQGPPGGPGIGPG    SEV GI PCPP YEFCGGMAYCGPQVG GLV
Sbjct  26   EPGWVDPLTWLRFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGAGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE  482
            PQ G+ET QPEG+AG  VESNS+G S EPC   P AVKL  EK+E  PEESQD+KALQKE
Sbjct  86   PQGGLETSQPEGEAGVGVESNSDGASPEPCTVPPGAVKLEKEKLEQNPEESQDIKALQKE  145

Query  483  LE  488
            LE
Sbjct  146  LE  147


>ref|XP_002746363.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Callithrix 
jacchus]
Length=360

 Score =  133 bits (334),  Expect = 1e-34
 Identities = 94/122 (77%), Positives = 100/122 (82%), Gaps = 6/122 (5%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            E GWVDPRTWLSFQGPPGGPGIGPG    +EV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct  26   ETGWVDPRTWLSFQGPPGGPGIGPGVGPGAEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE  482
            PQ G+ET QPEG+AGA VESNSEG S EPC   P AVKL  EK+E   EESQD+KALQKE
Sbjct  86   PQGGLETSQPEGEAGAGVESNSEGASPEPCTIPPGAVKLEKEKLEQNTEESQDIKALQKE  145

Query  483  LE  488
            LE
Sbjct  146  LE  147


>ref|NP_001108427.1| POU domain, class 5, transcription factor 1 [Macaca mulatta]
Length=360

 Score =  133 bits (334),  Expect = 1e-34
 Identities = 94/122 (77%), Positives = 100/122 (82%), Gaps = 6/122 (5%)
 Frame = +3

Query  141  EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv  308
            E GWVDPRTWLSFQGPPGGPGIGPG    SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct  26   ETGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV  85

Query  309  pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE  482
            PQ G+ET QPEG+AGA VESNS+G S EPC     AVKL  EK+E  PEESQD+KALQKE
Sbjct  86   PQGGLETSQPEGEAGAGVESNSDGASPEPCTVPTGAVKLEKEKLEQNPEESQDIKALQKE  145

Query  483  LE  488
            LE
Sbjct  146  LE  147



Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 104017620564


  Database: NCBI Protein Reference Sequences
    Posted date:  Mar 18, 2012  8:41 PM
  Number of letters in database: 4,140,237,112
  Number of sequences in database:  11,879,989



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 12
Window for multiple hits: 40
