BLASTP 2.2.26+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: minirefseq_prot
           20 sequences; 6,406 total letters



Query= random_s00

Length=32


***** No hits found *****



Lambda     K      H
   0.399    0.202    0.884 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 156650


Query= gi|16080617|ref|NP_391444.1| membrane bound lipoprotein [Bacillus
subtilis subsp. subtilis str. 168]

Length=102
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  gi|308175296|ref|YP_003922001.1| membrane bound lipoprotein [Ba...   139    2e-46
  gi|375363999|ref|YP_005132038.1| lytA gene product [Bacillus am...  89.0    7e-27
  gi|154687679|ref|YP_001422840.1| LytA [Bacillus amyloliquefacie...  89.0    8e-27
  gi|311070071|ref|YP_003974994.1| unnamed protein product [Bacil...  83.2    1e-24
  gi|332258565|ref|XP_003278367.1| PREDICTED: UPF0764 protein C16...  15.8    7.1  


> gi|308175296|ref|YP_003922001.1| membrane bound lipoprotein [Bacillus 
amyloliquefaciens DSM 7]
Length=100

 Score =  139 bits (350),  Expect = 2e-46, Method: Compositional matrix adjust.
 Identities = 69/102 (68%), Positives = 81/102 (79%), Gaps = 2/102 (2%)

Query  1    MKKFIALLFFILLLSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVDNEPV  60
            MKK    LFFILLL+GCGV ++KSQGED      + TKEGTYVGLADTHTIEVTVD+EPV
Sbjct  1    MKKIFGCLFFILLLAGCGVTNEKSQGEDAG--EKLVTKEGTYVGLADTHTIEVTVDHEPV  58

Query  61   SLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERAN  102
            S DITEES  D+   N+G+KVT+ Y+KN +GQL+LKDIE AN
Sbjct  59   SFDITEESADDVKNLNNGEKVTVKYQKNSKGQLVLKDIEPAN  100


> gi|375363999|ref|YP_005132038.1| lytA gene product [Bacillus 
amyloliquefaciens subsp. plantarum CAU B946]
Length=105

 Score = 89.0 bits (219),  Expect = 7e-27, Method: Compositional matrix adjust.
 Identities = 48/105 (46%), Positives = 69/105 (66%), Gaps = 5/105 (5%)

Query  1    MKKFIALLFFILL----LSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVD  56
            MKK IA  F ILL    L+ CG   Q  +G   S ++  + +   YVG+ADTHTIEV VD
Sbjct  1    MKKTIAASFLILLFSVVLAACGTAEQSKKGSG-SSENQAQKETAYYVGMADTHTIEVKVD  59

Query  57   NEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERA  101
            ++PVS + +++ +  L+KF+  DKV+ITY  ND+GQ  +K+IE+A
Sbjct  60   DQPVSFEFSDDFSDVLNKFSENDKVSITYFTNDKGQKEIKEIEKA  104


> gi|154687679|ref|YP_001422840.1| LytA [Bacillus amyloliquefaciens 
FZB42]
Length=105

 Score = 89.0 bits (219),  Expect = 8e-27, Method: Compositional matrix adjust.
 Identities = 48/105 (46%), Positives = 69/105 (66%), Gaps = 5/105 (5%)

Query  1    MKKFIALLFFILL----LSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVD  56
            MKK IA  F ILL    L+ CG   Q  +G   S ++  + +   YVG+ADTHTIEV VD
Sbjct  1    MKKTIAASFLILLFSVVLAACGTADQSKKGSG-SSENQAQKETAYYVGMADTHTIEVKVD  59

Query  57   NEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIERA  101
            ++PVS + +++ +  L+KF+  DKV+ITY  ND+GQ  +K+IE+A
Sbjct  60   DQPVSFEFSDDFSDVLNKFSENDKVSITYFTNDKGQKEIKEIEKA  104


> gi|311070071|ref|YP_003974994.1| unnamed protein product [Bacillus 
atrophaeus 1942]
Length=105

 Score = 83.2 bits (204),  Expect = 1e-24, Method: Compositional matrix adjust.
 Identities = 45/104 (43%), Positives = 66/104 (63%), Gaps = 5/104 (5%)

Query  1    MKKFIALLFFILL----LSGCGVNSQKSQGEDVSPDSNIETKEGTYVGLADTHTIEVTVD  56
            MKK +A  F ILL    L+ CG   Q  +G + S  S ++ +   YVG+ADTHTIEV +D
Sbjct  1    MKKNVASSFLILLFSIILAACGTAEQSKEG-NGSSSSQVQNETAYYVGMADTHTIEVKID  59

Query  57   NEPVSLDITEESTSDLDKFNSGDKVTITYEKNDEGQLLLKDIER  100
            ++PVS + T++ +  L++F   DKV I+Y  ND+GQ  L +IE+
Sbjct  60   DQPVSFEFTDDFSEILNEFEENDKVNISYLTNDKGQKELTEIEK  103


> gi|332258565|ref|XP_003278367.1| PREDICTED: UPF0764 protein C16orf89-like 
[Nomascus leucogenys]
Length=132

 Score = 15.8 bits (29),  Expect = 7.1, Method: Compositional matrix adjust.
 Identities = 7/25 (28%), Positives = 11/25 (44%), Gaps = 0/25 (0%)

Query  60   VSLDITEESTSDLDKFNSGDKVTIT  84
            V +       + L+   SGD  T+T
Sbjct  80   VEMGFLHVGQAGLELVTSGDPPTLT  104



Lambda     K      H
   0.310    0.131    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 361344


Query= gi|11464971:4-101 pleckstrin [Mus musculus]

Length=98
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  gi|11464971|ref|NP_062422.1| pleckstrin [Mus musculus]               205    2e-69
  gi|354480464|ref|XP_003502426.1| PREDICTED: pleckstrin-like [Cr...   205    3e-69
  gi|156616273|ref|NP_002655.2| pleckstrin [Homo sapiens]              204    1e-68
  gi|297667453|ref|XP_002811995.1| PREDICTED: pleckstrin-like [Po...   204    1e-68
  gi|350596020|ref|XP_003360649.2| PREDICTED: pleckstrin-like [Su...   199    2e-68
  gi|33188429|ref|NP_872601.1| histone demethylase UTY isoform 1 ...  18.5    0.88 
  gi|57113895|ref|NP_001009002.1| histone demethylase UTY [Pan tr...  18.5    0.88 


> gi|11464971|ref|NP_062422.1| pleckstrin [Mus musculus]
Length=350

 Score =  205 bits (522),  Expect = 2e-69, Method: Compositional matrix adjust.
 Identities = 98/98 (100%), Positives = 98/98 (100%), Gaps = 0/98 (0%)

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  4    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  63

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  64   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  101


 Score = 43.5 bits (101),  Expect = 3e-09, Method: Compositional matrix adjust.
 Identities = 29/100 (29%), Positives = 48/100 (48%), Gaps = 6/100 (6%)

Query  3    IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS--PCQD  58
            I++G L+K+G     WK    +L ED   + +Y       P G + L+G  +TS     D
Sbjct  246  IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAVHLRGCVVTSVESSHD  305

Query  59   FGK--RMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
              K     + +I T  +  ++ QAA  +ER  W++ I+ A
Sbjct  306  VKKSDEENLFEIITADEVHYYLQAATSKERTEWIKAIQVA  345


> gi|354480464|ref|XP_003502426.1| PREDICTED: pleckstrin-like [Cricetulus 
griseus]
Length=350

 Score =  205 bits (521),  Expect = 3e-69, Method: Compositional matrix adjust.
 Identities = 98/98 (100%), Positives = 98/98 (100%), Gaps = 0/98 (0%)

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  4    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  63

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  64   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  101


 Score = 43.9 bits (102),  Expect = 2e-09, Method: Compositional matrix adjust.
 Identities = 30/100 (30%), Positives = 50/100 (50%), Gaps = 6/100 (6%)

Query  3    IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDF-  59
            I++G L+K+G     WK    +L ED   + +Y       P G I L+G  +TS   +  
Sbjct  246  IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNHD  305

Query  60   GKRM---FVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
            GK+     + +I T  +  ++ QAA  +ER  W++ I+ A
Sbjct  306  GKKSDDENLFEIITADEVHYYLQAAAPKERTEWIKAIQVA  345


> gi|156616273|ref|NP_002655.2| pleckstrin [Homo sapiens]
Length=350

 Score =  204 bits (518),  Expect = 1e-68, Method: Compositional matrix adjust.
 Identities = 97/98 (99%), Positives = 97/98 (99%), Gaps = 0/98 (0%)

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  4    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  63

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  64   KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  101


 Score = 47.4 bits (111),  Expect = 2e-10, Method: Compositional matrix adjust.
 Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 6/100 (6%)

Query  3    IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            I++G L+K+G     WK    +L ED   + +Y       P G I L+G  +TS   +  
Sbjct  246  IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN  305

Query  61   KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
             R      + +I T  +  +F QAA  +ER  W+R I+ A
Sbjct  306  GRKSEEENLFEIITADEVHYFLQAATPKERTEWIRAIQMA  345


> gi|297667453|ref|XP_002811995.1| PREDICTED: pleckstrin-like [Pongo 
abelii]
Length=350

 Score =  204 bits (518),  Expect = 1e-68, Method: Compositional matrix adjust.
 Identities = 97/98 (99%), Positives = 97/98 (99%), Gaps = 0/98 (0%)

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  4    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  63

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  64   KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  101


 Score = 45.4 bits (106),  Expect = 6e-10, Method: Compositional matrix adjust.
 Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)

Query  3    IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            I++G L+K+G     WK    +L ED   + +Y       P G I L+G  +TS   +  
Sbjct  246  IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN  305

Query  61   KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
             R      + +I T  +  +F QAA  +ER  W++ I+ A
Sbjct  306  GRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA  345


> gi|350596020|ref|XP_003360649.2| PREDICTED: pleckstrin-like [Sus 
scrofa]
Length=228

 Score =  199 bits (506),  Expect = 2e-68, Method: Compositional matrix adjust.
 Identities = 94/98 (96%), Positives = 96/98 (98%), Gaps = 0/98 (0%)

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGS+FNTWKPMWV+LLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  4    KRIREGYLVKKGSMFNTWKPMWVILLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  63

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERD WVRDIKKAIK
Sbjct  64   KRMFVFKITTTKQQDHFFQAAFLEERDGWVRDIKKAIK  101



Lambda     K      H
   0.321    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 345626


  Database: minirefseq_prot
    Posted date:  Jun 9, 2012  2:05 PM
  Number of letters in database: 6,406
  Number of sequences in database:  20



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
