TBLASTN 2.2.26+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: minirefseq_mrna
           23 sequences; 67,750 total letters



Query= random_s00

Length=32


***** No hits found *****





Effective search space used: 0


Query= gi|16080617|ref|NP_391444.1| membrane bound lipoprotein [Bacillus
subtilis subsp. subtilis str. 168]

Length=102
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  gi|145479850|ref|XM_001425911.1| Paramecium tetraurelia hypothe...  34.7    1e-05
  gi|72012412|ref|XM_777959.1| PREDICTED: Strongylocentrotus purp...  31.6    1e-04
  gi|115975252|ref|XM_001180111.1| PREDICTED: Strongylocentrotus ...  31.6    1e-04


> gi|145479850|ref|XM_001425911.1| Paramecium tetraurelia hypothetical 
protein (GSPATT00004923001) partial mRNA
Length=4632

 Score = 34.7 bits (78),  Expect = 1e-05, Method: Composition-based stats.
 Identities = 15/43 (35%), Positives = 26/43 (60%), Gaps = 0/43 (0%)
 Frame = +1

Query  31    PDSNIETKEGTYVGLADTHTIEVTVDNEPVSLDITEESTSDLD  73
             P +   TK+GT +GL   HTI   + +  +SL++ E++  D+D
Sbjct  1744  PKTATGTKKGTIIGLLSIHTILFILTSHALSLEVKEQT*KDID  1872


> gi|72012412|ref|XM_777959.1| PREDICTED: Strongylocentrotus purpuratus 
hypothetical LOC577746 (LOC577746), mRNA
Length=1593

 Score = 31.6 bits (70),  Expect = 1e-04, Method: Composition-based stats.
 Identities = 20/59 (34%), Positives = 29/59 (49%), Gaps = 8/59 (14%)
 Frame = +1

Query  44    GLADTHTIEVTVDNEPVSLDITEESTSDLDKFNSG--------DKVTITYEKNDEGQLL  94
             GL   HT+ + V +    LD+TEE  ++LD+F S          K  + YE+N   Q L
Sbjct  1057  GLVPDHTLILPVGHYQSMLDLTEEVQTELDQFKSALRKYYLSKGKTCVIYERNFRTQHL  1233


> gi|115975252|ref|XM_001180111.1| PREDICTED: Strongylocentrotus 
purpuratus hypothetical LOC577746 (LOC577746), mRNA
Length=1593

 Score = 31.6 bits (70),  Expect = 1e-04, Method: Composition-based stats.
 Identities = 20/59 (34%), Positives = 29/59 (49%), Gaps = 8/59 (14%)
 Frame = +1

Query  44    GLADTHTIEVTVDNEPVSLDITEESTSDLDKFNSG--------DKVTITYEKNDEGQLL  94
             GL   HT+ + V +    LD+TEE  ++LD+F S          K  + YE+N   Q L
Sbjct  1057  GLVPDHTLILPVGHYQSMLDLTEEVQTELDQFKSALRKYYLSKGKTCVIYERNFRTQHL  1233



Lambda     K      H
   0.302    0.125    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 1205400


Query= gi|11464971:4-101 pleckstrin [Mus musculus]

Length=98
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  gi|350596019|ref|XM_003360601.2| PREDICTED: Sus scrofa pleckstr...   199    2e-67
  gi|301779869|ref|XM_002925302.1| PREDICTED: Ailuropoda melanole...   202    2e-67
  gi|296223671|ref|XM_002757683.1| PREDICTED: Callithrix jacchus ...   202    4e-67
  gi|338714227|ref|XM_001492113.3| PREDICTED: Equus caballus plec...   202    2e-66
  gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS 42...  19.6    1.7  
  gi|94721341|ref|NM_001040441.1| Homo sapiens zinc finger and BT...  17.7    6.3  
  gi|332865370|ref|XM_527768.3| PREDICTED: Pan troglodytes zinc f...  17.3    8.7  
  gi|332865372|ref|XM_003318468.1| PREDICTED: Pan troglodytes zin...  17.3    8.7  


> gi|350596019|ref|XM_003360601.2| PREDICTED: Sus scrofa pleckstrin-like 
(LOC100626968), mRNA
Length=772

 Score =  199 bits (506),  Expect = 2e-67, Method: Compositional matrix adjust.
 Identities = 94/98 (96%), Positives = 96/98 (98%), Gaps = 0/98 (0%)
 Frame = +2

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGS+FNTWKPMWV+LLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  95   KRIREGYLVKKGSMFNTWKPMWVILLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  274

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERD WVRDIKKAIK
Sbjct  275  KRMFVFKITTTKQQDHFFQAAFLEERDGWVRDIKKAIK  388


 Score = 32.7 bits (73),  Expect = 4e-05, Method: Compositional matrix adjust.
 Identities = 21/71 (30%), Positives = 33/71 (46%), Gaps = 4/71 (6%)
 Frame = +2

Query  30   IEFYKKKSDNSPKGMIPLKGSTLTS-PCQDFGKRMFVLK---ITTTKQQDHFFQAAFLEE  85
            + +Y       P G I L+G  +TS      GK  F+ +     T  +  +F QAA  +E
Sbjct  542  LHYYDPAGGEDPLGAIHLRGCVVTSVESNTDGKNGFLWERAXXITADEVHYFLQAANPKE  721

Query  86   RDAWVRDIKKA  96
            R  W++ I+ A
Sbjct  722  RTEWIKAIQVA  754


> gi|301779869|ref|XM_002925302.1| PREDICTED: Ailuropoda melanoleuca 
pleckstrin-like (LOC100466932), mRNA
Length=1144

 Score =  202 bits (515),  Expect = 2e-67, Method: Compositional matrix adjust.
 Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
 Frame = +3

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  78   KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  257

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  258  KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  371


 Score = 45.1 bits (105),  Expect = 3e-09, Method: Compositional matrix adjust.
 Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
 Frame = +3

Query  3     IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC  56
             I++G L+K+G     WK    +L ED   + +Y       P G I L+G  +TS    P 
Sbjct  804   IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNPD  983

Query  57    QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
                 +   + +I T  +  +F QAA  +ER  W++ I+ A
Sbjct  984   VRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA  1103


> gi|296223671|ref|XM_002757683.1| PREDICTED: Callithrix jacchus 
pleckstrin (PLEK), mRNA
Length=1183

 Score =  202 bits (515),  Expect = 4e-67, Method: Compositional matrix adjust.
 Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
 Frame = +2

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVKKGS+FNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  161  KRIREGYLVKKGSMFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  340

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  341  KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  454


 Score = 45.1 bits (105),  Expect = 3e-09, Method: Compositional matrix adjust.
 Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
 Frame = +2

Query  3     IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
             I++G L+K+G     WK    +L ED   + +Y       P G I L+G  +TS   +  
Sbjct  866   IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNSD  1045

Query  61    KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
              R      + +I T  +  +F QAA  +ER  W++ I+ A
Sbjct  1046  GRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA  1165


> gi|338714227|ref|XM_001492113.3| PREDICTED: Equus caballus pleckstrin-like 
(LOC100051039), mRNA
Length=1390

 Score =  202 bits (515),  Expect = 2e-66, Method: Compositional matrix adjust.
 Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
 Frame = +2

Query  1    KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  60
            KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct  173  KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG  352

Query  61   KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  98
            KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct  353  KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK  466


 Score = 46.6 bits (109),  Expect = 1e-09, Method: Compositional matrix adjust.
 Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
 Frame = +2

Query  3     IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC  56
             I++G L+K+G     WK    VL ED   + +Y       P G I L+G  +TS    P 
Sbjct  899   IKQGCLLKQGHRRKNWKVRKFVLREDPAYVHYYDPAGGEEPLGAIHLRGCVVTSVEGNPD  1078

Query  57    QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA  96
                 +   + +I T  +  +F QAA  +ER  W++ I+ A
Sbjct  1079  GKKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA  1198


> gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS 
421 hypothetical protein (NDAI0A06120), mRNA
Length=4932

 Score = 19.6 bits (39),  Expect = 1.7, Method: Compositional matrix adjust.
 Identities = 16/52 (31%), Positives = 23/52 (44%), Gaps = 9/52 (17%)
 Frame = +1

Query  12    GSVFNTWKPMWVVLL---------EDGIEFYKKKSDNSPKGMIPLKGSTLTS  54
             GS F TW  +++ +L         E   E   K  D + KG++ L  S  TS
Sbjct  3181  GSCFPTWDLIFIEVLNPFLKEKLWEADNEEISKFVDLTLKGLVDLYPSHFTS  3336



Lambda     K      H
   0.321    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 1119300


  Database: minirefseq_mrna
    Posted date:  Jun 9, 2012  2:05 PM
  Number of letters in database: 67,750
  Number of sequences in database:  23



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
