BLASTP 2.0.11 [Jan-20-2000]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
         (197 letters)

Database: data/swissprot
           82,258 sequences; 29,652,561 total letters

Searching..................................................done


Results from round 1


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN       402  e-112
gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN I...    30  3.3
gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE                      29  7.4
gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 1...    29  7.4

>gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
           Length = 197
           
 Score =  402 bits (1021), Expect = e-112
 Identities = 197/197 (100%), Positives = 197/197 (100%)

Query: 1   MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
           MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 1   MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60

Query: 61  FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
           FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 61  FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120

Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
           TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180

Query: 181 RRFADRLMVKLNHIRIK 197
           RRFADRLMVKLNHIRIK
Sbjct: 181 RRFADRLMVKLNHIRIK 197


>gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN IN
           NMD5-HOM6 INTERGENIC REGION
           Length = 1442
           
 Score = 30.1 bits (66), Expect = 3.3
 Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 10/70 (14%)

Query: 107 TLMASKETLLKR--------LRTRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQ 158
           TL+  K+  L R          TR + K S AA   D+ +EGLS P    +  +D  +  
Sbjct: 637 TLLTRKDPSLSRNLKQSAGDALTRKQEKRSKAA--FDQLLEGLSGPPLHVYYASDGGNAA 694

Query: 159 DVAENIAARA 168
           ++A+ +AARA
Sbjct: 695 NLAKRLAARA 704


>gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE
          Length = 376
          
 Score = 29.0 bits (63), Expect = 7.4
 Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 2/37 (5%)

Query: 3  IWINGAFGSGKTQTAFELHRRLNP--SYVYDPEKMGF 37
          ++I+G  G GKT T+ +L   L P  + VY PE M +
Sbjct: 52 VYIDGPHGVGKTTTSAQLMEALGPRDNIVYVPEPMTY 88


>gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 190
           KD POLYPEPTIDE
           Length = 1689
           
 Score = 29.0 bits (63), Expect = 7.4
 Identities = 12/38 (31%), Positives = 20/38 (52%)

Query: 75  GILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASK 112
           G +++P+   HP +F+++   LR      HHF L   K
Sbjct: 78  GHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVK 115


Searching..................................................done


Results from round 2


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value
Sequences used in model and found again:

gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN       406  e-113

Sequences not found previously or not previously below threshold:

gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN I...    30  3.3
gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 1...    30  3.3
gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE                      29  7.4


CONVERGED!
>gi|1174726|sp|P12921|TMRB_BACSU TUNICAMYCIN RESISTANCE PROTEIN
           Length = 197
           
 Score =  406 bits (1031), Expect = e-113
 Identities = 197/197 (100%), Positives = 197/197 (100%)

Query: 1   MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60
           MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA
Sbjct: 1   MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60

Query: 61  FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120
           FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR
Sbjct: 61  FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120

Query: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180
           TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL
Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180

Query: 181 RRFADRLMVKLNHIRIK 197
           RRFADRLMVKLNHIRIK
Sbjct: 181 RRFADRLMVKLNHIRIK 197


>gi|1352934|sp|P47169|YJ9F_YEAST HYPOTHETICAL 161.2 KD PROTEIN IN
           NMD5-HOM6 INTERGENIC REGION
           Length = 1442
           
 Score = 30.1 bits (66), Expect = 3.3
 Identities = 23/70 (32%), Positives = 35/70 (49%), Gaps = 10/70 (14%)

Query: 107 TLMASKETLLKR--------LRTRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQ 158
           TL+  K+  L R          TR + K S AA   D+ +EGLS P    +  +D  +  
Sbjct: 637 TLLTRKDPSLSRNLKQSAGDALTRKQEKRSKAA--FDQLLEGLSGPPLHVYYASDGGNAA 694

Query: 159 DVAENIAARA 168
           ++A+ +AARA
Sbjct: 695 NLAKRLAARA 704


>gi|3334489|sp|P15398|RPA1_SCHPO DNA-DIRECTED RNA POLYMERASE I 190
           KD POLYPEPTIDE
           Length = 1689
           
 Score = 30.1 bits (66), Expect = 3.3
 Identities = 12/38 (31%), Positives = 20/38 (52%)

Query: 75  GILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASK 112
           G +++P+   HP +F+++   LR      HHF L   K
Sbjct: 78  GHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVK 115


>gi|3915741|sp|P04407|KITH_HSV23 THYMIDINE KINASE
          Length = 376
          
 Score = 28.9 bits (63), Expect = 7.4
 Identities = 15/37 (40%), Positives = 22/37 (58%), Gaps = 2/37 (5%)

Query: 3  IWINGAFGSGKTQTAFELHRRLNP--SYVYDPEKMGF 37
          ++I+G  G GKT T+ +L   L P  + VY PE M +
Sbjct: 52 VYIDGPHGVGKTTTSAQLMEALGPRDNIVYVPEPMTY 88


  Database: data/swissprot
    Posted date:  Feb 2, 2000  9:39 AM
  Number of letters in database: 29,652,561
  Number of sequences in database:  82,258
  
Lambda     K      H
   0.318    0.134    0.412 

Lambda     K      H
   0.270   0.0471    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23669955
Number of Sequences: 82258
Number of extensions: 918938
Number of successful extensions: 2130
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2126
Number of HSP's gapped (non-prelim): 8
length of query: 197
length of database: 29,652,561
effective HSP length: 48
effective length of query: 149
effective length of database: 25,704,177
effective search space: 3829922373
effective search space used: 3829922373
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 62 (28.6 bits)
