TBLASTX 2.2.26+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: minirefseq_mrna
           23 sequences; 67,750 total letters



Query= random_s00

Length=128


***** No hits found *****





Effective search space used: 0


Query= gi|296147483:1-350 Saccharomyces cerevisiae S288c Mon2p (MON2) mRNA,
complete cds

Length=350
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value  N

  gi|296147483|ref|NM_001183135.1| Saccharomyces cerevisiae S288c...   289    2e-81  1
  gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS 42...   152    5e-42  2
  gi|366988334|ref|XM_003673886.1| Naumovozyma castellii CBS 4309...   143    3e-37  1
  gi|255710474|ref|XM_002551475.1| Lachancea thermotolerans CBS 6...   141    8e-37  1
  gi|254579534|ref|XM_002495708.1| Zygosaccharomyces rouxii hypot...   141    1e-36  1
  gi|350596019|ref|XM_003360601.2| PREDICTED: Sus scrofa pleckstr...  25.4    0.091  1
  gi|332865372|ref|XM_003318468.1| PREDICTED: Pan troglodytes zin...  23.5    0.33   1
  gi|145479850|ref|XM_001425911.1| Paramecium tetraurelia hypothe...  21.7    1.2    1
  gi|94721341|ref|NM_001040441.1| Homo sapiens zinc finger and BT...  21.2    1.3    2
  gi|332237160|ref|XM_003267724.1| PREDICTED: Nomascus leucogenys...  19.9    4.1    1


> gi|296147483|ref|NM_001183135.1| Saccharomyces cerevisiae S288c 
Mon2p (MON2) mRNA, complete cds
Length=4911

 Score =  289 bits (626),  Expect = 2e-81
 Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
 Frame = +2/+2

Query  2    WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISH  181
            WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISH
Sbjct  2    WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISH  181

Query  182  SLSCWLANQGMLK*RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K  349
            SLSCWLANQGMLK*RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K
Sbjct  182  SLSCWLANQGMLK*RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K  349


 Score =  278 bits (602),  Expect = 5e-78
 Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
 Frame = -2/-3

Query  349  LLIESPSRDE*PQ*RHPKFQTAGFEE*MERLTVPVGIALPGSSF*HSLIGKPTRKGVRNP  170
            LLIESPSRDE*PQ*RHPKFQTAGFEE*MERLTVPVGIALPGSSF*HSLIGKPTRKGVRNP
Sbjct  349  LLIESPSRDE*PQ*RHPKFQTAGFEE*MERLTVPVGIALPGSSF*HSLIGKPTRKGVRNP  170

Query  169  DVFLAPQNYVLFSISQWIYH*HGEWLNFFFSIRKIKNAILLQVAFAWSQTLQCSWP  2
            DVFLAPQNYVLFSISQWIYH*HGEWLNFFFSIRKIKNAILLQVAFAWSQTLQCSWP
Sbjct  169  DVFLAPQNYVLFSISQWIYH*HGEWLNFFFSIRKIKNAILLQVAFAWSQTLQCSWP  2


 Score =  274 bits (593),  Expect = 8e-77
 Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
 Frame = -3/-1

Query  348  F*LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIR  169
            F*LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIR
Sbjct  348  F*LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIR  169

Query  168  MSF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH  1
            MSF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH
Sbjct  168  MSF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH  1


 Score =  270 bits (583),  Expect = 2e-75
 Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
 Frame = +3/+3

Query  3    GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIEKST*F*GARKTSGFRT  182
            GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIEKST*F*GARKTSGFRT
Sbjct  3    GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIEKST*F*GARKTSGFRT  182

Query  183  PFRVGLPIKEC*NDDPGNAMPTGTVNRSIYSSKPAV*NFGCLH*GYSSRDGDSIKS  350
            PFRVGLPIKEC*NDDPGNAMPTGTVNRSIYSSKPAV*NFGCLH*GYSSRDGDSIKS
Sbjct  183  PFRVGLPIKEC*NDDPGNAMPTGTVNRSIYSSKPAV*NFGCLH*GYSSRDGDSIKS  350


 Score =  229 bits (495),  Expect = 3e-63
 Identities = 116/116 (100%), Positives = 116/116 (100%), Gaps = 0/116 (0%)
 Frame = -1/-2

Query  350  TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTKgsaks  171
            TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTKGSAKS
Sbjct  350  TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTKGSAKS  171

Query  170  gclssssklCTLFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESNPPVFMA  3
            GCLSSSSKLCTLFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESNPPVFMA
Sbjct  170  GCLSSSSKLCTLFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESNPPVFMA  3


 Score =  197 bits (425),  Expect(2) = 2e-58
 Identities = 85/85 (100%), Positives = 85/85 (100%), Gaps = 0/85 (0%)
 Frame = +1/+1

Query  94   TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI  273
            TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI
Sbjct  94   TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI  273

Query  274  PRSRLSEILDAFIEATHLAMEIQLK  348
            PRSRLSEILDAFIEATHLAMEIQLK
Sbjct  274  PRSRLSEILDAFIEATHLAMEIQLK  348


 Score = 36.3 bits (73),  Expect(2) = 2e-58
 Identities = 14/14 (100%), Positives = 14/14 (100%), Gaps = 0/14 (0%)
 Frame = +1/+1

Query  1   MAMNTGGFDSMQRQ  42
           MAMNTGGFDSMQRQ
Sbjct  1   MAMNTGGFDSMQRQ  42


 Score = 18.9 bits (35),  Expect = 7.8
 Identities = 6/11 (55%), Positives = 9/11 (82%), Gaps = 0/11 (0%)
 Frame = +2/-3

Query  293  KFWMPSLRLLI  325
            K W+P ++LLI
Sbjct  373  KKWVPPVKLLI  341


> gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS 
421 hypothetical protein (NDAI0A06120), mRNA
Length=4932

 Score =  152 bits (327),  Expect(2) = 5e-42
 Identities = 62/85 (73%), Positives = 73/85 (86%), Gaps = 0/85 (0%)
 Frame = +1/+1

Query  94   TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI  273
            TI+HASDKSI+ILK + + EEL RHPDF  P VLAC SRNAK+T++AMQCLQGL++VPSI
Sbjct  88   TIKHASDKSIDILKTIQNIEELVRHPDFVTPLVLACSSRNAKLTSIAMQCLQGLASVPSI  267

Query  274  PRSRLSEILDAFIEATHLAMEIQLK  348
            P SR+ E+LD FIEAT LAMEIQLK
Sbjct  268  PESRIPEVLDGFIEATQLAMEIQLK  342


 Score = 26.3 bits (51),  Expect(2) = 5e-42
 Identities = 11/14 (79%), Positives = 11/14 (79%), Gaps = 0/14 (0%)
 Frame = +3/+3

Query  69   FRIEKKKFNHSPC*  110
            FRI KKKFNH  C*
Sbjct  63   FRI*KKKFNH*TC*  104


 Score = 68.0 bits (142),  Expect(2) = 1e-24
 Identities = 34/55 (62%), Positives = 38/55 (69%), Gaps = 0/55 (0%)
 Frame = -1/-2

Query  350  TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTK  186
            TFN ISIA *VASM  S+ S     GI+GT  +PCRHC+A  V LAFLD QA TK
Sbjct  344  TFNCISIAS*VASMNPSRTSGILDSGIEGTDANPCRHCMAIEVNLAFLDEQARTK  180


 Score = 52.8 bits (109),  Expect(2) = 1e-24
 Identities = 24/39 (62%), Positives = 29/39 (74%), Gaps = 0/39 (0%)
 Frame = -1/-2

Query  137  LFNISMDLSLAWRMVEFLLFDSEDKERNSASSCLCMESN  21
            +F IS+DLSLA  MVEFLL DSE++E NS S+C   E N
Sbjct  131  VFRISIDLSLACLMVEFLLLDSEERECNSWSNCFRKEVN  15


 Score = 61.1 bits (127),  Expect = 2e-12
 Identities = 36/103 (35%), Positives = 52/103 (50%), Gaps = 0/103 (0%)
 Frame = +2/+2

Query  41   NLKQNCVLYLPNRKEEIQPFAMLVINPLRY*KEYIVLRS*KDIRISHSLSCWLANQGMLK  220
            N  +N   +L N KEEIQP  ML  N   +*+ + + ++     I   L  W  ++ M  
Sbjct  35   NWTRNYTPFLQNLKEEIQPLNMLATNQSIF*RLFKI*KNL*GTLILLHLWSWPVHREMPN  214

Query  221  *RPWQCNAYRDCQPFHLFLEAGCLKFWMPSLRLLISRWRFN*K  349
            *    CNAY+D +P+  +      KF   SL+ L  RWR+N*K
Sbjct  215  *LQLPCNAYKDWRPYLQYQSQESQKF*TDSLKPLS*RWRYN*K  343


 Score = 42.8 bits (87),  Expect = 5e-07
 Identities = 28/65 (43%), Positives = 36/65 (55%), Gaps = 0/65 (0%)
 Frame = -3/-1

Query  261  G*QSL*ALHCQGRHFSIP*LASQHERECEIRMSF*LLKTMYSFQYLNGFITSMANG*ISS  82
            G QSL*ALH     F I     Q +R  +IR+ +     + S   ++ F+ SM NG*ISS
Sbjct  255  GRQSL*ALHGN*S*FGISR*TGQDQRCNKIRVPYKFFYILNSL*NID*FVASMFNG*ISS  76

Query  81   FRFGR  67
            FRF R
Sbjct  75   FRF*R  61


 Score = 24.0 bits (46),  Expect(2) = 0.028
 Identities = 11/17 (65%), Positives = 11/17 (65%), Gaps = 0/17 (0%)
 Frame = -2/-3

Query  118  IYH*HGEWLNFFFSIRK  68
            I  *H  WLNFFF I K
Sbjct  112  ICR*HV*WLNFFF*ILK  62


 Score = 23.5 bits (45),  Expect(2) = 0.028
 Identities = 9/17 (53%), Positives = 11/17 (65%), Gaps = 0/17 (0%)
 Frame = -3/-2

Query  153   LKTMYSFQYLNGFITSM  103
             L   +SF + NGF TSM
Sbjct  3263  LSASHSFSFKNGFNTSM  3213


 Score = 21.7 bits (41),  Expect = 1.2
 Identities = 9/30 (30%), Positives = 16/30 (53%), Gaps = 0/30 (0%)
 Frame = +2/-2

Query  167  IRISHSLSCWLANQGMLK*RPWQCNAYRDC  256
            I I  SL+C +    +L     +CN++ +C
Sbjct  122  ISIDLSLACLMVEFLLLDSEERECNSWSNC  33


 Score = 18.9 bits (35),  Expect = 7.8
 Identities = 6/11 (55%), Positives = 9/11 (82%), Gaps = 0/11 (0%)
 Frame = +3/+3

Query  306  LH*GYSSRDGD  338
            +H* +S+ DGD
Sbjct  300  IH*SHSASDGD  332


> gi|366988334|ref|XM_003673886.1| Naumovozyma castellii CBS 4309 
hypothetical protein (NCAS0A09950) mRNA, complete cds
Length=4938

 Score =  143 bits (306),  Expect = 3e-37
 Identities = 58/85 (68%), Positives = 71/85 (84%), Gaps = 0/85 (0%)
 Frame = +1/+1

Query  94   TIRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSI  273
            +I+HASDKSIEILK + + E+L  HPDF  P V +C SRNAK+T++AMQCLQGL++ PSI
Sbjct  94   SIKHASDKSIEILKTIQNIEDLASHPDFVTPLVESCLSRNAKLTSIAMQCLQGLASAPSI  273

Query  274  PRSRLSEILDAFIEATHLAMEIQLK  348
            P SRLS +LD FIEATHLA+EIQLK
Sbjct  274  PESRLSGVLDGFIEATHLAIEIQLK  348


 Score = 62.5 bits (130),  Expect(2) = 1e-20
 Identities = 30/54 (56%), Positives = 36/54 (67%), Gaps = 0/54 (0%)
 Frame = -1/-2

Query  350  TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANT  189
            TF+ ISIAR VAS+  S+  DS L G+DG    PC+HCIA  V LAFLD   +T
Sbjct  350  TFSCISIARCVASINPSRTPDSLLSGMDGADAKPCKHCIAIEVNLAFLDRHDST  189


 Score = 44.6 bits (91),  Expect(2) = 1e-20
 Identities = 20/33 (61%), Positives = 24/33 (73%), Gaps = 0/33 (0%)
 Frame = -1/-2

Query  137  LFNISMDLSLAWRMVEFLLFDSEDKERNSASSC  39
            +F IS+DLSLA  +  FLL DSED E NS S+C
Sbjct  137  VFKISIDLSLACLIEVFLLLDSEDNECNSRSNC  39


 Score = 54.2 bits (112),  Expect = 2e-10
 Identities = 38/115 (33%), Positives = 58/115 (50%), Gaps = 0/115 (0%)
 Frame = -3/-1

Query  345  *LNLHREMSSLNEGIQNFRQPASRNRWNG*QSL*ALHCQGRHFSIP*LASQHERECEIRM  166
            *L  + +M    + I+N R  A R  W    +L* LH      S+P*    ++   +IRM
Sbjct  345  *LYFNCKMCRFYKSIENSR*SAFRYGWCRC*AL*TLHSNRGQLSVP**T*FYQWSDKIRM  166

Query  165  SF*LLKTMYSFQYLNGFITSMANG*ISSFRFGR*RTQFCFKLPLHGVKPSSVHGH  1
            +  +   + SF  L+  +T M +  + SFRFGR   QF  +L L G+K  S++ H
Sbjct  165  ACQIFNVLDSF*DLDRLVTCMLDRSVPSFRFGRQ*MQF*VQLFLKGLKTCSLNSH  1


 Score = 29.5 bits (58),  Expect = 0.005
 Identities = 18/45 (40%), Positives = 22/45 (49%), Gaps = 0/45 (0%)
 Frame = +2/+2

Query  2    WP*TLEGLTPCKGNLKQNCVLYLPNRKEEIQPFAMLVINPLRY*K  136
            W   L+ L P K N   NC+  LPN KE     +M V +  R *K
Sbjct  2    WLFKLQVLRPFKNNWT*NCIHCLPNLKEGTLLSSMQVTSLSRS*K  136


 Score = 29.0 bits (57),  Expect = 0.007
 Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 0/44 (0%)
 Frame = +3/+3

Query  3    GHEHWRV*LHAKAT*SRIAFFIFRIEKKKFNHSPC***IH*DIE  134
            G  ++R *  +K    RIAF +FRI KK+  +  C * ++ D++
Sbjct  3    GCSNYRF*DLSKTIGPRIAFIVFRI*KKEHFYQACK*QVYRDLK  134


 Score = 22.1 bits (42),  Expect = 0.84
 Identities = 10/17 (59%), Positives = 11/17 (65%), Gaps = 0/17 (0%)
 Frame = -2/-3

Query  85  FFSIRKIKNAILLQVAF  35
           FF IRK  NAIL  + F
Sbjct  85  FF*IRKTMNAILGPIVF  35


 Score = 20.3 bits (38),  Expect = 3.0
 Identities = 6/13 (46%), Positives = 8/13 (62%), Gaps = 0/13 (0%)
 Frame = +2/-2

Query  278   EAGCLKFWMPSLR  316
             +  CL FW+ S R
Sbjct  4217  QTSCLNFWVMSSR  4179


> gi|255710474|ref|XM_002551475.1| Lachancea thermotolerans CBS 
6340 KLTH0A01342p (KLTH0A01342g) mRNA, complete cds
Length=4845

 Score =  141 bits (303),  Expect = 8e-37
 Identities = 55/84 (65%), Positives = 71/84 (85%), Gaps = 0/84 (0%)
 Frame = +1/+1

Query  97   IRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIP  276
            +RHASDKSIEILK VH FE+L RHPDF  PFVL+C S+NAK+T++++QCLQ +S+V  IP
Sbjct  94   VRHASDKSIEILKTVHEFEDLPRHPDFVTPFVLSCASKNAKLTSVSVQCLQKMSSVNCIP  273

Query  277  RSRLSEILDAFIEATHLAMEIQLK  348
              R+ ++LDAFI++THLA EIQLK
Sbjct  274  EDRIEDVLDAFIDSTHLAAEIQLK  345


 Score = 45.1 bits (92),  Expect = 1e-07
 Identities = 25/55 (45%), Positives = 29/55 (53%), Gaps = 0/55 (0%)
 Frame = -1/-2

Query  350  TFN*ISIAR*VASMKASKISDSRLRGIDGTVDSPCRHCIARVVILAFLDWQANTK  186
            T + IS AR V SM AS  S     G+  T D  CRHC   +V LAFL+    TK
Sbjct  347  TLSCISAARWVESMNASSTSSILSSGMQLTDDIFCRHCTETLVSLAFLEAHERTK  183


 Score = 29.0 bits (57),  Expect = 0.007
 Identities = 22/70 (31%), Positives = 33/70 (47%), Gaps = 0/70 (0%)
 Frame = -3/-1

Query  249  L*ALHCQGRHFSIP*LASQHERECEIRMSF*LLKTMYSFQYLNGFITSMANG*ISSFRFG  70
            L*ALH      S+     + ER  ++ M + + + M SFQ L   +T M +   +  R  
Sbjct  246  L*ALH*DACELSVFGGTRKDERRYKVWMPWKVFEFMNSFQDLY*LVTCMPHLRAAPLRLR  67

Query  69   R*RTQFCFKL  40
            R   QFC +L
Sbjct  66   RKCVQFCEEL  37


 Score = 21.2 bits (40),  Expect = 1.6
 Identities = 6/8 (75%), Positives = 7/8 (88%), Gaps = 0/8 (0%)
 Frame = +2/+2

Query  233  QCNAYRDC  256
            QCNAY+ C
Sbjct  230  QCNAYKRC  253


 Score = 18.9 bits (35),  Expect = 7.8
 Identities = 5/10 (50%), Positives = 6/10 (60%), Gaps = 0/10 (0%)
 Frame = -2/-2

Query  31    WSQTLQCSWP  2
             WS  +  SWP
Sbjct  2951  WSDIMLISWP  2922


> gi|254579534|ref|XM_002495708.1| Zygosaccharomyces rouxii hypothetical 
protein (ZYRO0C02266g) mRNA, complete cds
Length=4866

 Score =  141 bits (302),  Expect = 1e-36
 Identities = 57/84 (68%), Positives = 72/84 (86%), Gaps = 0/84 (0%)
 Frame = +1/+1

Query  97   IRHASDKSIEILKRVHSFEELERHPDFALPFVLACQSRNAKMTTLAMQCLQGLSTVPSIP  276
            IR+ASDKSIEILK VHS+EEL RHPDF +P V++C S+NAK+TT++MQC Q L+TVP IP
Sbjct  97   IRNASDKSIEILKVVHSYEELSRHPDFIVPLVMSCASKNAKLTTISMQCFQKLATVPCIP  276

Query  277  RSRLSEILDAFIEATHLAMEIQLK  348
              +LS++LDAFIEA  LAM+I+LK
Sbjct  277  VDKLSDVLDAFIEANQLAMDIKLK  348



Lambda     K      H
   0.318    0.134    0.401 


Effective search space used: 1954618


  Database: minirefseq_mrna
    Posted date:  Jun 9, 2012  2:05 PM
  Number of letters in database: 67,750
  Number of sequences in database:  23



Matrix: BLOSUM62
Neighboring words threshold: 13
Window for multiple hits: 40
